Enzymes and Inhibitors
Thermo Scientific™ FastDigest FokI
Cut at GGATG(N)9^ sites with Thermo Scientific™ FastDigest™ FokI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest NheI
Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Klenow Fragment (10 U/μL)
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
Thermo Scientific™ ApaI (10 U/μL)
Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).
Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
Thermo Scientific™ Eco91I (BstEII) (10 U/μL)
The Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer (Isoschizomers: BstEII, BstPI, EcoO65I, PspEI).
Thermo Scientific™ FastDigest Eam1105I
Cut at GACNNN^NNGTC sites with Thermo Scientific™ FastDigest™ Eam1105I, which performs best using universal FastDigest Buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).
Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.
Thermo Scientific™ FastDigest FaqI
Cut at GGGAC(10/14)^ sites with Thermo Scientific™ FastDigest™ BsmFI (FaqI), which performs best using universal FastDigest Buffer (Isoschizomers: BslFI).
Thermo Scientific™ FastDigest OliI
Cut at CACNN^NNGTG sites with Thermo Scientific™ FastDigest™ AleI (OliI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ Bsp68I (NruI) (10 U/μL)
Cut at TCG^CGA sites with Bsp68I (NruI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BtuMI, NruI).
Thermo Scientific™ Eco88I (AvaI) (10 U/μL)
Cut at C^YCGRG sites with Eco88I (AvaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: Ama87I, AvaI, BmeT110I, BsiHKCI, BsoBIm).
Thermo Scientific™ KpnI (10 U/μL)
The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).