Enzymes and Inhibitors
Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
Thermo Scientific™ BamHI (10 U/μL)
The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ Eco31I (BsaI) (10 U/μL)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Thermo Scientific™ FastDigest XmaJI
Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI).
Thermo Scientific™ FastDigest BcuI
FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).
Thermo Scientific™ Tru1I (MseI) (10 U/μL)
Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).
Thermo Scientific™ SmiI (SwaI) (10 U/μL)
Cut at ATTT^AAAT sites with SmiI (SwaI) restriction enzyme, which performs best at 30°C in O buffer (Isoschizomers: SwaI).
Thermo Scientific™ SgrDI (5 U/μL)
Cut at CG^TCGACG sites with SgrDI restriction enzyme, which functions best at 37°C in R buffer.
Thermo Scientific™ MssI (PmeI) (5 U/μL)
The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Thermo Scientific™ MreI (Sse232I) (10 U/μL)
Cut at CG^CCGGCG sites with MreI (Sse232I) restriction enzyme, which performs best at 37°C in G buffer.
Thermo Scientific™ FastDigest NsbI
Cut at TGC^GCA sites with Thermo Scientific™ FastDigest™ FspI (NsbI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc16I, AviII).
Thermo Scientific™ FastDigest PstI
The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).
Thermo Scientific™ FastDigest PvuII
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.